The Rainbow River is located in the southwest corner of Marion County Florida. It is formed by a first magnitude spring that is ranked fourth in the state for volume of discharge. In addition to the springs located at the headwaters, there are many smaller springs that discharge from numerous caves, rock crevices, and sand boils the entire length of the river. The Rainbow River is a gently winding river that is 5.7 miles long and merges with the Withlacoochee River at Dunnellon Florida. The headwaters is the anchor for the Rainbow Springs State Park. This first magnitude spring is not one large vent but is numerous vents that issue 400 - 600 million gallons of crystalline water every day.
Rainbow Springs State Park is comprised of 1,459.07 upland acres (which includes around 100 acres of wetlands) and 12.83 submerged acres. The most significant natural feature is the first magnitude headspring basin which produces between 400 - 600 million gallons of fresh water per day, forming the Rainbow River. The looking glass waters of Rainbow Springs come from several vents, not one large bubbling spring. The river itself supports a wide variety of fish , wildlife, and plants, many within easy viewing by visitors. In total, the park contains 11 distinct natural communities, including sandhills, flatwoods, upland mixed forests, and hydric hammocks. Visitors are able to see a variety of wildflowers in season; oak, longleaf pines, magnolia, dogwood, redbud, and hickory trees; gray squirrels, red-shoulder hawks, swallowtail kites, barred owls, whitetail deer, and a wide variety of wading birds. The relative peace and quiet of the winter season offers much for the nature enthusiast. There is an interpretive room located in the visitor center displaying historical, natural, and cultural resources of the park.